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Google Scholar Citations:
JA Schoborg, MC Jewett (2018) “Cell‐Free Protein Synthesis: An Emerging Technology for Understanding, Harnessing, and Expanding the Capabilities of Biological Systems” Synthetic Biology: Parts, Devices and Applications 301-330
Karim AS and Jewett MC (2018) “Cell-free synthetic biology for pathway prototyping” Methods in Enzymology. Accepted.
d’Aquino AE, Kim DS, and Jewett MC (2018) “Engineered ribosomes for basic science and synthetic biology.” Annual Review of Chemical and Biomolecular Engineering. Online.
Wang H, Li J, and Jewett MC (2018) “Development of a Pseudomonas putida cell-free protein synthesis platform for rapid screening of gene regulatory elements” Synthetic Biology. 3(1): ysy003
Casini A, Chang F-Y, Eluere R, King A, Young E, Dudley QM, Karim A, Pratt K, Bristol C, Forget A, Ghodasara A, Warden-Rothman R, Gan R, Cristofaro A, Borujeni AE, Ryu M, Li J, Kwon YC, Wang H, Tatsis E, Rodriguez-Lopez C, O’Connor S, Medema MH, Fischbach MA, Jewett MC, Voigt C, Gordon DB (2018) “A Pressure Test to Make 10 Molecules in 90 Days: External Evaluation of Methods to Engineer Biology” J. Am. Chem. Soc. 140 (12): 4302-4316
Kightlinger W, Lin L, Rosztoczy M, DeLisa MP, Mrksich M, and Jewett MC (2018) “Design of glycosylation sites by rapid expression and high-throughput characterization of N- glycosyltransferase” Nature Chemical Biology. Online.
Martin RW, Des Soye BJ, Kwon YC, Kay J, Davis RG, Thomas PM, Marcum RD, Majewska NI, Chen CX, Weiss MG, Stoddart AE, Amiram M, Charna AK, Isaacs FJ, Kelleher NL, Hong SH, and Jewett MC (2018) “Cell-free protein synthesis from recoded bacteria enables multi-site non-canonical amino acid incorporation at high yield and purity” Nature Communications 9: 1203
Kakkar N, Perez JG, Liu W, Jewett MC, van der Donk W (2018) “Incorporation of non-proteinogenic amino acids in class I and II lantibiotics.” ACS Chemical Biology.
Aebersold R, Agar JN, Amster J, Baker M, Bertozzi CR, Boja ES, Costello CE, Cravatt BF, Fenselau C, Garcia B, Ge Y, Gunawardena J, Hendrickson RC, Hergenrother PJ, Huber C, Ivanov AR, Jensen O, Jewett MC, Kelleher NL, Kiessling LL, Krogan NJ, Larsen MR, Loo JA, Loo RR, Lundberg E, MacCoss M, Mallick P, Mootha VK, Mrksich M, Muir TW, Patrie SM, Pesavento JJ, Pitteri SJ, Rodriguez H, Saghatelian A, Sandoval W, Schlüter H, Sechi A, Sheeley DM, Slavoff SA, Smith LM, Snyder MP, Thomas PM, Uhlen M, van der Donk WA, van Eyk JE, Vidal M, Walt DR, White FM, Williams ER, Wohlschlager T, Wysocki VH, Yates NA, Young NL, Zhang B (2018) “How many human proteoforms are there?” Nature Chemical Biology 14(3): 206-214.
Liu SS, Hockenberry AJ, Lancichinetti A, Jewett MC, Amaral LAN (2018) “A novel framework for evaluating the performance of codon usage bias metrics.” Journal of the Royal Society Interface 15: 20170667
Liu CC, Jewett MC, Chin JW, and Voigt CA (2018) “Towards an orthogonal central dogma” Nature Chemical Biology 14:103-106
Schoborg JA, Hershewe J, Stark JC, Kightlinger W, Kath JE, Jaroentomeechai T, Natarajan A, DeLisa MP, and Jewett MC (2018) “A cell-free platform for rapid synthesis and testing of active oligosaccharyltransferases ” Biotechnology and Bioengineering 115(3): 739-750
Li J, Wang H, and Jewett MC (2018) “Expanding the palette of Streptomyces-based cell-free protein synthesis systems with enhanced yields” Biochemical Engineering Journal 130: 29-33
Hockenberry AJ, Stern AJ, Amaral LAN, and Jewett MC (2018) “Diversity of translation initiation mechanisms across bacterial species is driven by environmental conditions and growth demands” Molecular Biology and Evolution 35 (3): 582-592
Karim AS, Heggestad JT, Crowe SA, and Jewett MC (2018) ” Controlling cell-free metabolism through physiochemical perturbations ” Metabolic Engineering 45: 86-94
Martin RW, Majewska NI, Chen CX, Albanetti TE, Jimenez RB, Schmelzer AE, Jewett MC, Roy V (2017) “Development of a CHO-based Cell-free Platform for Synthesis of Active Monoclonal Antibodies” ACS Synthetic Biology 6(7): 1370-1379
Li J, Haas W, Jackson K, Kuru E, Jewett MC, Fan ZH, Gygi SP, and Church GM (2017) “Co-generating synthetic parts toward a self-replicating system” ACS Synthetic Biology 6(7): 1370-1379
Jaroentomeechai T, Zheng X, Hershewe J, Stark JC, Jewett MC, and DeLisa MP (2017) “A pipeline for interrogating and engineering single-subunit oligosaccharyltransferases” Methods in Enzymology, Chemical Glycobiology. 597: 55-81
Liu Y, Kim DS, and Jewett MC (2017) “Repurposing ribosomes for synthetic biology” Current Opinion in Chemical Biology. 40: 87-94
Li J, Wang H, Kwon YC, Jewett MC (2017) “Establishing a High Yielding Streptomyces-BasedCell-Free Protein Synthesis System” Biotechnology and Bioengineering 114(6), 1343-1353
Hockenberry AJ, Pah AR, Jewett MC, Amaral LAN (2017) “Leveraging genome-wide datasets to quantify the functional role of the anti-Shine–Dalgarno sequence in regulating translation efficiency” Open Biology 7(1), 160239
Gan R, Perez JG, Carlson ED, Ntai I, Isaacs FJ, Kelleher NL, Jewett MC (2017) “Translation system engineering in Escherichia coli enhances non‐canonical amino acid incorporation into proteins” Biotechnology and Bioengineering 114(5), 1074-1086
Liu SS, Hockenberry AJ, Jewett MC, Amaral LAN (2016) “NullSeq: A Tool for Generating Random Coding Sequences with Desired Amino Acid and GC Contents” PLoS Computional Biology 12(11), e1005184
Yang C, Hockenberry AJ, Jewett MC, Amaral LAN (2016) “Depletion of Shine-Dalgarno sequences within bacterial coding regions is expression dependent” G3: Genes| Genomes| Genetics 6(11), 3467-3474
Goering AW, Li J, McClure RA, Thomson RJ, Jewett MC, and Kelleher NL (2016) “In vitro Reconstruction of Nonribosomal Peptide Biosynthesis Directly from DNA Using Cell-Free Protein Synthesis” ACS Synthetic Biology 6, 39-44
Dudley QM, Anderson KC, and Jewett MC (2016) “Cell-free mixing of Escherichia coli crude extracts to prototype and rationally engineer high-titer mevalonate synthesis” ACS Synthetic Biology 5(12), 1578-1588
Karim AS and Jewett MC (2016) “A cell-free framework for rapid biosynthetic pathway prototyping and enzyme discovery” Metabolic Engineering 36, 116-126
Schoborg JA†, Clark LG†, Choudhury A†, Hodgman CE†, and Jewett MC (2016) “Yeast knockout library allows for efficient testing of genomic mutations for cell-free protein synthesis” Synthetic and Systems Biotechnology 1:1, 2-6
Caschera F, Lee JW, Ho KKY, Liu AP, and Jewett MC (2016) “Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with in vitro synthesized and assembled ribosomes” Chemical Communications 52:31, 5467-5469
Gan R and Jewett MC (2016) “Evolution of translation initiation sequences using in vitro yeast ribosome display” Biotechnology and Bioengineering In press
Li J, Lawton TJ, Kostecki JS, Nisthal A, Fang J, Mayo SL, Rosenzweig AC, and Jewett, MC (2016) “Cell-free protein synthesis enables high yielding synthesis of an active multicopper oxidase” Biotechnology Journal 11, 212–218
Amiram M, Haimovich AD, Fan C, Wang YS, Aerni HR, Ntai I, Moonan DW, Ma NJ, Rovner AJ, Hong SH, Kelleher NL, Goodman AL, Jewett MC, Söll D, Rinehart J, and Isaacs FJ (2015) “Evolution of translation machinery in recoded bacteria enables multi-site incorporation of nonstandard amino acids” Nature Biotechnology 33, 1272-1279
Sullivan CJ†, Pendleton ED†, Sasmor HH, Hicks WL, Farnum JB, Muto M, Amendt EM, Schoborg JA, Martin RW, Clark LG, Anderson MJ, Choudhury A, Fior R, Lo YH, Griffey RH, Chappell SA, Jewett MC, Mauro VP, and Dresios J “A cell-free expression and purification process for rapid production of protein biologics” Biotechnology journal In Press
Kay JE and Jewett MC (2015) “Lysate of engineered Escherichia coli supports high-level conversion of glucose to 2, 3-butanediol” Metabolic engineering 32, 133-142
Des Soye B, Patel J, Isaacs FJ, and Jewett MC (2015) “Repurposing the translation apparatus for synthetic biology” Current Opinion in Chemical Biology 28: 83-90
Oza JP,† Aerni HR† Pirman NL, Barber KW, ter Haar CM, Rogulina S, Amrofell MB, Isaacs FJ, Rinehart J, and Jewett MC (2015) “Robust production of recombinant phosphoproteins using cell-free protein synthesis” Nature Communications 6: 8168
Orelle C,† Carlson ED,† Szal T, Florin T, Jewett MC, and Mankin AS (2015) “Protein synthesis by ribosomes with tethered subunits” Nature 524: 119–124
Anderson MJ,† Stark JC,† Hodgman CE, and Jewett MC (2015) “Energizing yeast cell-free protein synthesis with glucose metabolism” FEBS Letters 589: 1723-1727
Moatsou D,† Li J,† Ranji A, Pitto-Barry A, Ntai I, Jewett MC, O’Reilly RK (2015) “Reversible self-assembly of temperature-responsive protein-polymer conjugates” Bioconjugate Chemistry 26: 1890-1899
Fritz BR, Jamil OK, and Jewett MC (2015) “Implications of macromolecular crowding and reducing conditions for in vitro ribosome construction” Nucleic Acids Research 43, 4774-4784
Heinzelman P, Schoborg JA, and Jewett MC (2015) “pH responsive granulocyte colony stimulating factor variants with implications for treating Alzheimer’s disease and other central nervous system disorders” Protein Engineering Design and Selection 28: 481-489
Hong SH,† Kwon YC,† Martin RW, Des Soye BJ, de Paz AM, Swonger KN, Ntai I, Kelleher NL, and Jewett MC (2015) “Improving Cell-free Protein Synthesis through Genome Engineering of Escherichia coli lacking Release Factor 1” ChemBioChem 16: 844-853 (inner cover)
Kwon YC and Jewett MC (2015) “High-throughput preparation methods of crude extract for robust cell-free protein synthesis” Scientific Reports 5, 8663
Ploskon E, Wagner S, Ellington AD, Jewett MC, O’Reilly R, and Booth P (2015) “Controlled assembly of artificial protein-protein complexes via DNA duplex formation” Bioconjugate Chemistry 26: 427–434
Dudley QM, Karim AS, and Jewett MC (2015) “Cell-Free Metabolic Engineering: Biomanufacturing beyond the cell” Biotechnology Journal 10: 69–82 (Review)
Choudury A,† Hodgman CE,† Anderson M, and Jewett MC (2014) “Evaluating fermentation effects on cell growth and crude extract metabolic activity for improved yeast cell-free protein synthesis” Biochemical Engineering Journal 91: 140-148
Hodgman CE and Jewett MC (2014) “Characterizing IGR IRES-mediatedtranslation initiation for use in yeast cell-free protein synthesis” New Biotechnology 31: 499-505
Liu Y, Fritz BR, Anderson MJ, Schoborg JA, and Jewett MC (2014) “Characterizing and alleviating substrate limitations for improved in vitro ribosome construction” ACS Synthetic Biology 4: 454-462
Fritz BR and Jewett MC (2014) “The impact of transcriptional tuning on in vitro integrated rRNA transcription and ribosome construction” Nucleic Acids Research 42: 6774-6785
Gan R and Jewett MC (2014) “A combined cell-free transcription-translationsystem from Saccharomyces cerevisiae for rapid and robust protein synthesis” Biotechnology Journal 9: 641-651
Schoborg JA,† Hodgman CE,† Anderson M, Jewett MC (2014) “Characterizing substrate limitations for improved yeast cell-free protein synthesis” Biotechnology Journal 9: 631-640
Hockenberry AJ,† Sirer MA,† Amaral LAN, and Jewett MC (2014) “Quantifying position dependent codon usage bias” Molecular Biology and Evolution 31: 1880-1893
Palmer MJ and Jewett MC (2014) “Enabling a next generation of synthetic biology community organization and leadership” ACS Synthetic Biology 3: 117–120 (Viewpoint)
Hong SH, Ntai I, Haimovich AD, Kelleher NL, Isaacs FJ, and Jewett MC (2014) “Cell-free protein synthesis from a release factor 1 deficient Escherichia coli activates efficient and multiple site-specific non-standard amino acid incorporation” ACS Synthetic Biology 3: 398-409
Hong SH, Kwon YC, and Jewett MC (2014) “Non-standard amino acid incorporation into proteins using Escherichia coli cell-free protein synthesis” Frontiers in Chemistry 2: 34
Jewett MC†, Workman CT†, Nookaew I, Pizarro FP, Hellgren L, and Nielsen J (2013) “Mapping Condition
Dependent Regulation of Lipid Metabolism” G3:Genes-Genomes-Genetics 3: 1979-1995
Hodgman CE, and Jewett MC (2013) “Optimizing extract preparation and reaction conditions to increase yield and reduce costs in a yeast cell-free protein synthesis system. Biotechnology and Bioengineering” 110: 2643-2654
Jewett MC, and Patolsky F (2013) “Nanobiotechnology: synthetic biology meets materials science” Current Opinion in Biotechnology 24: 551-554 (Editorial Overview)
Kang Y, Lu A, Ellington AD, Jewett MC, and O’Reilly RK (2013) “Effect of Complementary nucleobase interactions on the copolymer composition of RAFT copolymerizations” ACS Macro Letters 2: 581-586
Jewett MC, Fritz BR, Timmerman LE, and Church GM (2013) “In vitro integration of ribosomal RNA synthesis, ribosome assembly, and protein synthesis” Molecular Systems Biology 9: 678
Harris DC and Jewett MC (2012) “Cell-free biology: exploiting the interface between synthetic biology and synthetic chemistry” Current Opinion in Biotechnology 23: 672-678
Hockenberry AJ and Jewett MC (2012) “Synthetic in vitro circuits” Current Opinion in Chemical Biology 16: 261-269
Hodgman CE and Jewett MC (2012) “Cell-free synthetic biology: Thinking outside the cell” Metabolic Engineering 14: 261-269
Carlson ED, Gan R, Hodgman CE, and Jewett MC (2011) “Cell-free protein synthesis: Applications come of age” Biotechnology Advances 30: 1185-1194
Wang HH†, Huang P-Y†, Xu G, Haas W, Marblestone A, Li J, Gygi S, Forster AC, Jewett MC, and Church GM (2012) “In vivo tagging of enzyme ensembles with MAGE for in vitro single-pot multi-enzyme catalysis” ACS Synthetic Biology 1: 43-52
Isaacs FJ, Carr PA, Wang HH, Lajoie MJ, Sterling B, Kraal L, Tolonen A, Gianoulis T, Goodman D, Reppas NB, Emig CJ, Bang D, Hwang SJ, Jewett MC, Jacobson JM, and Church GM (2011) “Precise Manipulation of Chromosomes in vivo Enables Genome-wide Codon Replacement” Science 333: 348-353
Fritz BR, Timmerman LE, Daringer NM, Leonard JN, and Jewett MC (2010) “Biology by design: from top to bottom and back” Journal of Biomedicine and Biotechnology 232016.pdf
Jewett MC and Forster AC (2010) “Update on designing and building minimal cells” Current Opinion in Biotechnology 21: 697-703 (Review)
Moxley JF†, Jewett MC†, Antoniewicz MR†, Villas-Boas SG†, Alper HS, Wheeler RT, Tong L, Hinnebusch AG, Ideker T, Nielsen J, and Stephanopoulos GN (2009) “Linking high resolution metabolic flux phenotypes and transcriptional regulation in yeast modulated by the global regulator Gcn4p” PNAS 106: 6477-6482
Jewett MC, Miller ML, Chen Y, and Swartz JR (2009) “Continued protein synthesis at low [ATP] and [GTP] enables cell adaptation during starvation” Journal of Bacteriology 191: 1083-1091
Usaite R, Jewett MC, Oliveira AP, Yates JR, Olsson L, and Nielsen J (2009) “Reconstruction of the yeast Snf1 kinase regulatory network reveals its role as a global energy regulator” Molecular Systems Biology 5: 319
Pizarro FJ†, Jewett MC†, Nielsen J, and Agosin E (2008) “Physiological and transcriptional mapping of evolutionary differences between commercial and laboratory Saccharomyces cerevisiae strains” Applied Environmental Microbiology 74: 6358-6368
Nookaew N, Jewett MC, Meechai A, Thammarongtham C, Laoteng K, Cheevadhanarak S, Nielsen J, and Bhumiratana S (2008) “The genome-scale metabolic model iIN800 of Saccharomyces cerevisiae and its validation: a scaffold to query lipid metabolism” BMC Systems Biology 2: 71
Fazio A†, Jewett MC†, Daran-Lapujade P, Mustacchi R, Usaite R, Pronk JT, Workman CT, and Nielsen J (2008) “Transcription factor control of growth rate dependent genes in Saccharomyces cerevisiae: a three factor design” BMC Genomics 9: 341
Nielsen J., and Jewett, M.C. (2008) “The impact of systems biology and metabolic engineering of Saccharomyces cerevisiae” FEMS Yeast Research 8: 122-31 (Review)
Jewett MC, Calhoun KA, Voloshin A, Wuu JJ, and Swartz JR (2008) “An integrated cell-free metabolic platform for protein production and synthetic biology” Molecular Systems Biology 4: 220
Jewett MC, Hofmann G, and Nielsen J (2006) “Fungal metabolite analysis in genomics and phenomics” Current Opinion Biotechnology 17: 191-197 (Review)
Jewett MC, Oliveira AP, Patil KR, and Nielsen J (2005) “The role of high-throughput transcriptome analysis in metabolic engineering” Biotechnology and Bioprocess Engineering 10: 385-399 (Review)
Jewett MC, and Swartz JR (2004) “Substrate replenishment extends protein synthesis with an in vitro system designed to mimic the cytoplasm” Biotechnology and Bioengineering 87: 465-472
Jewett MC, and Swartz JR (2004) “Mimicking the Escherichia coli cytoplasmic environment activates long-lived and efficient protein synthesis” Biotechnology and Bioengineering 86: 19-26
JR Swartz, MC Jewett, KA Woodrow 2004. Cell-free protein synthesis with prokaryotic combined transcription-translation Recombinant Gene Expression, 169-182
Jewett MC, and Swartz JR (2004) “Rapid expression and purification of 100 nmol quantities of active protein using cell-free protein synthesis” Biotechnology Progress 20: 102-109
Perez, J.G.,† Stark, J.C.,† and Jewett, M.C.* 2015. Cell-free synthetic biology: Engineering beyond the cell, in “Synthetic Biology,” J. Craig Venter, Daniel Gibson, Hamilton Smith, Clyde Hutchison, editors. Cold Spring Harbor Laboratory Press. Accepted/In Press.
Karim, A.S., Dudley, Q.M, and Jewett, M.C.* 2015. Cell-free synthetic systems for metabolic engineering and biosynthetic pathway prototyping, in “Industrial Biotechnology,” James C. Liao, editor. Wiley Biotechnology Series. In Press.
Schoborg, J.A. and Jewett, M.C.* 2015. Cell-free protein synthesis: an emerging technology for understanding, harnessing, and expanding the capabilities of biological systems, in “Synthetic Biology,” Sven Panke and Christina Smolke, editors. Wiley Biotechnology Series. In press.
Ranji, A., Wu, J.C., Bundy, B.C.*, and Jewett M.C.* 2013. Transforming synthetic biology with cell-free systems, in “Synthetic Biology”, Huimin Zhao, editor. Waltham, MA, Elsevier. p. 277-301.
Oliveira, A.P., Jewett, M.C., and Nielsen, J.* 2007. From gene expression to metabolic fluxes. In: Choi S, editor. Introduction to Systems Biology. Totowa, NJ: Humana Press Inc. p. 37-68.
Nielsen J.* and Jewett, M.C. 2007. The role of metabolomics in systems biology. In: Nielsen J, Jewett M.C., editors. Metabolomics: a powerful tool in systems biology. Topics in Current Genetics Series. Heidelberg, Germany: Springer. p. 1-10.
Jewett, M.C., Hansen, M.E., and Nielsen J.* 2007. Data acquisition, analysis, and mining: Integrative tools for discerning metabolic function in Saccharomyces cerevisiae. In: Nielsen J, Jewett M.C., editors. Metabolomics: a powerful tool in systems biology. Topics in Current Genetics Series. Heidelberg, Germany: Springer. p. 159-187.
Swartz, J.R.*, Jewett, M.C., and Woodrow, K.A. 2004. Cell-free protein synthesis with prokaryotic combined transcription-translation. In: Balbas P, Lorence A, editors. Recombinant Protein Protocols: Methods in Molecular Biology Series: Volume 267. Totowa, NJ: Humana Press Inc. p. 169-182.
Jewett, M.C., Voloshin, A., and Swartz, J.R.* 2002. Prokaryotic systems for in vitro Expression. In: Weiner MP, Lu Q, editors. Gene Cloning and Expression Technologies. Westborough, MA: Eaton Publishing. p. 391-411.
Swartz, J.R. and Jewett, M.C. 2013. Improved Methods of In Vitro Protein Synthesis. Canada Patent No. 2,496,437.
Calhoun, K.A., Jewett, M.C., and Swartz, J.R. 2013. Improved Methods of In Vitro Protein Synthesis. Korean Patent No.: 10-1232656.
Swartz, J.R. and Jewett, M.C. 2013. Methods of in vitro protein synthesis. U.S. Patent No.: 8,357,529.
Bond, R., Jewett, M.C., McAuliffe, J.C., and Ward, D.E. 2010. Biocomposite comprising co-precipitate of enzyme, silicate and polyamine. U.S. Patent Number 7,642,077.
Swartz, J.R. and Jewett, M.C. 2010. A method for synthesis of polynucleotides and/or polypeptides. India Patent No.: 239129.
Calhoun, K.A., Jewett, M.C., and Swartz, J.R. 2009. Improved Methods of In Vitro Protein Synthesis. Australian Patent No.: 2004293798.
Swartz, J.R. and Jewett, M.C. 2009. Improved Methods of In Vitro Protein Synthesis. Australian Patent No.: 2003259912.
Calhoun, K.A., Jewett, M.C., and Swartz, J.R. 2009. Improved Methods of In Vitro Protein Synthesis. New Zealand Patent No.: 546961.
Swartz, J.R. and Jewett, M.C. 2009. Improved Methods of In Vitro Protein Synthesis. European Patent No.: 1539948.
Swartz, J.R. and Jewett, M.C. 2008. Methods of in vitro protein synthesis. U.S. Patent No.: 7,338,789.